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KMID : 0545120090190010051
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 1 p.51 ~ p.54
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LC-MS/MS Profiling-Based Secondary Metabolite Screening of Myxococcus xanthus
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Kim Ji-Young
Choi Jung-Nam
Kim Pil
Sok Dai-Eun
Nam Soo-Wan
Lee Choong-Hwan
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Abstract
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Myxobacteria, Gram-negative soil bacteria, are a well-known producer of bioactive secondary metabolites. Therefore, this study presents a methodological approach for the high-throughput screening of secondary metabolites from 4 wild-type Myxococcus xanthus strains. First, electrospray ionization mass spectrometry (ESI-MS) was performed using extracellular crude extracts. As a result, 22 metabolite peaks were detected, and the metabolite profiling was then conducted using the m/z value, retention time, and MS/MS fragmentation pattern analyses. Among the peaks, one unknown compound peak was identified as analogous to the myxalamid A, B, and C series. An analysis of the tandem mass spectrometric fragmentation patterns and HR-MS identified myxalamid K as a new compound derived from M. xanthus. In conclusion, LC-MS/MS-based chemical screening of diverse secondary metabolites would appear to be an effective approach for discovering unknown microbial secondary metabolites.
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KEYWORD
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Metabolite profiling, LC-MS/MS, Myxococcus
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